OTHER TOOLS AND RESOURCES
3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI)
4H-Imidazol-4-one, 5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-, 5 (Z)- / 1241390-29-3
Absorption and fluorescence emission spectra of DFHBI in pH 7.4 buffer.
PRESENTATION: Lyophilized dye
STORAGE CONDITIONS: Stable in the dark for 2 year at -20 ºC.
QUALITY ASSURANCE: Products are analyzed by 1H NMR and LC-MS and provided at purity of >95% by HPLC.
USAGE STATEMENT: This product is intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Due to the highly specific nature of fluorophores and aptamers, we cannot predict or be held responsible with respect to how Lucerna products will behave in its customers' systems. Researchers using Lucerna products should conduct optimization studies to achieve the optimal result possible for their intended application.
LICENSING: Cornell University has filed patents titled "RNA sequences that switch on the fluorescence of small molecules and their use in detection of RNA in vitro and in cells" and "novel methods for RNA detection and quantification". All commercial users should contact Cornell University for a use license.
HEK293T cells were transfected with either 5S-control aptamer or 5S-Spinach plasmids and incubated with 20 µM DFHBI. Images are phase overlay with Hoechst-stained nuclei (blue) and Spinach fluorescence (green).
Spinach RNA robustly activates the fluorescence of DFHBI. Each tube contained the indicated solution and was irradiated at 465 nm.
REFERENCE FOR THE PRODUCT:
RNA mimics of green fluorescent protein. Paige JS, Wu KY, Jaffrey SR. Science. 2011 Jul 29;333(6042):642-6.
THIS PRODUCT WAS CITED IN:
The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology. Pothoulakis G, Ceroni F, Reeve B, Ellis T. ACS Syn Biol. 2013 Sep 13.
A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA. Strack RL, Disney MD, Jaffrey SR. Nat Mehtods. 2013 Dec; 10(12):1219-24.
Gene position more strongly influences cell-free protein expression from operons than T7 transcriptional promoter strength. Chizzolini F, Forlin M, Cecchi D, Mansy SS. ACS Syn Biol. 2013 Dec 9.