OTHER TOOLS AND RESOURCES
(Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T)
4H-Imidazol-4-one, 5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-, (5Z) / 1539318-36-0
Absorption and fluorescence emission spectra of Spinach2TM/DFHBI-1T in pH 7.4 buffer.
PRESENTATION: Lyophilized dye
STORAGE CONDITIONS: Stable in the dark for 2 year at -20 ºC.
QUALITY ASSURANCE: Products are analyzed by 1H NMR and LC-MS and provided at purity of >95% by HPLC.
USAGE STATEMENT: This product is intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Due to the highly specific nature of fluorophores and aptamers, we cannot predict or be held responsible with respect to how LucernaTM products will behave in its customers' systems. Researchers using LucernaTM products should conduct optimization studies to achieve the optimal result possible for their intended application.
LICENSING: Cornell University has filed patents titled "RNA sequences that switch on the fluorescence of small molecules and their use in detection of RNA in vitro and in cells" and "novel methods for RNA detection and quantification." All commercial users should contact Cornell University for a use license.
A. Live-cell imaging of a COS7 cell expressing CGG60-Spinach2TM in the presence of either 20 µM DFHBI or DFHBI-1T. Images of the same cell were acquired using a 100 msec exposure with a GFP filter set. B. Quantification of average DFHBI-1T brightness of 10 cells expressing CGG60-SpinachTM normalized to the brightness of DFHBI.
REFERENCE FOR THE PRODUCT:
Song W, Strack RL, Svensen N, Jaffrey SR. 2014. Plug-and-Play Fluorophores Extend the Spectral Properties of Spinach. JACS136(4):1198-1201.
THIS PRODUCT WAS CITED IN:
Svensen N, Jaffrey SR. 2016. Fluorescent RNA aptamers as a tool to study RNA-modifying enzymes. Cell Chem Biol 23(3):415-25.
Aw SS, Tang, MX, Teo YN, Cohen SM. 2016. A conformation-induced fluorescence method for microRNA detection. Nucleic Acids Res 44(10):e92.
Alam KK, Tawiah KD, Lichte MF, Porciani, Burke DH. 2017. A fluorescent split aptamer for visualizing RNA-RNA assembly in vivo. ACS Synth Biol 6(9):1710-1721.
Lucerna has the exclusive rights to all patents covering the use of this product. All commercial users should contact Lucerna for a use license.